Biochemistry Unit

Policlonal Antibodies

Protein Expression and Purification

We provide both custom services...:

• Ag-affinity purification of polyclonal antibodies from crude antisera
• Protein G / Protein A affinity purification of monoclonal antibodies from serum free media
• Protein crosslinking to agarose-sepharose beads
• Antibody labeling with biotine or fluorocromes (several Abs/emission spectra available from Alexa)
• Customized protocol for antisera production c/o a selected Company. Coordination of all the immunization steps (from the order to the stocking). SUBMIT A REQUEST (only for Campus members)
• Ordering of free, modified and conjugated peptides. SUBMIT A REQUEST (only for Campus members)

... and common services:

• Ordering and central stocking of the Campus protein network reagents. SEE THE LIST (only for Campus members)

Prior to place whatever antibody/purification/modification request, we suggest to contact the Facility at the address service-abcifom-ieo-campus.it to discuss and plan your needs. Optimization for each request is almost necessary due to the characteristics of each technique and purpose.