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Biochemistry Unit

Cogentech (Consortium for Genomic Technologies)
c/o IFOM-IEO Campus
Via Adamello, 16 - 20139 Milan, Italy
The Facility offers integrated services for the production and characterization of the proteins.
What does it mean for the user and what kind of problems may solve for him?
  1. Protein production (in E. coli and insect cells, in the next future in P. pastoris).
  2. Monoclonal and recombinant antibody production
  3. Protein/antibody labeling/tagging.
  4. Biochemical characterization (ITC/ CD/ Fluorimeter, Biacore 2000).
  5. Production of enzymes of general use (TEV, 3C, Taq, Pfu, T7, LIF, Cre).
  6. Conservation of vector and strain collections.
  7. Collection of protocols and general advice.
  8. Organization of workshops and practical courses.
  9. Test of new commercial products and collaboration with private companies for the development of new technologies.
  10. Development research for the optimization of the protein production (co-expression of molecular chaperones, use of chemical chaperones, optimization of in vivo and in vitro re-folding).
  11. Development of specific "user-driven" technologies (in collaboration with the research groups).

Therefore, we could produce your protein, use part of it for producing antibodies (monoclonals or recombinant), then label both antigen and antibodies for LM or EM applications, and in the meanwhile control their folding and measure their binding constants, check for the polymerization state of the protein and its interactions with other macromolecules or substrates. Such a service is not only aimed at speeding up the work of non-specialists but to improve the quality of their results in terms of reproducibility, precision and completeness of the data.

last update: February 14, 2012 . Copyright © IFOM & IEO . Campus IFOM-IEO . Via Adamello 16 . 20139 Milan Italy
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