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Mass Spectrometry Unit

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Coordinator: Gabriela Grigorean
Cogentech (Consortium for Genomic Technologies)
c/o IFOM-IEO Campus
Via Adamello, 16 - 20139 Milan, Italy

staff:
Paolo Soffientini
Andrea Di Fonzo
Theodora Manousidou
tel. + 39 02 574303 706/5
fax. + 39 02 574303 231
service-proteomicsmailifom-ieo-campus.it

Activities

The Mass Spectrometry Facility offers proteomics services on a fee basis, and are interested in research collaborations with both investigators at IFOM-IEO Campus and outside. We provide assistance in scientific and technical matters necessary for a proteomics experiment. This includes assistance in pre-analysis set-up (i.e., how to do a mass-spec "compatible" IP, gel), and post-analysis assistance such as data interpretation. We accept in-gel or in-liquid samples. Analysis can be either/both via electrospray (ESI) and matrix-assisted laser desorption (MALDI) ionization techniques. We also offer gel facilities, use of a prep-LC, mass spectrometry-based protein quantitation, using iTRAQ technology, analysis of SILAC-labeled samples and AQUA quantitation (user provides labeled peptides).

Services offered:

Instrumentation:

1. an Ultra LTQ-FT, Thermo Electron, on-line with a 1100 Agilent nanoLC, for 1-dimensional RP HPLC separation prior to the mass spec analysis.

instrument 01 [enlarge]

 

2. Q-Star XL, Applied Biosystems, on-line with a 1100 Agilent nanoLC, for 1-dimensional RP HPLC separation prior to the mass spec analysis.

instrument 02[enlarge]

 

3. 4800 MALDI TOF/TOF (AB)

instrument 03[enlarge]

 

4. Dionex nano-LC coupled to a MALDI spotter

5. prep-scale 1200 Agilent HPLC, currently used for SCX

update: Oct 2010
 
more info about this unit:
 
last update: February 14, 2012 . Copyright © IFOM & IEO . Campus IFOM-IEO . Via Adamello 16 . 20139 Milan Italy
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