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Imaging Unit

Dario Parazzoli (Facility Director)
Cogentech (Consortium for Genomic Technologies)
c/o IFOM-IEO Campus, Via Adamello, 16 - 20139 Milan, Italy
dario.parazzolimailifom-ieo-campus.it

Our Mission

The Imaging Center provides access to optical technology for biomedical research. The provided services cover all the main needs of basic and advanced cancer research: flow cytometry and cell sorting , cell microinjection, wide field and confocal fluorescence microscopy, time-lapse microscopy, and digital image analysis basic and advanced applications (deconvolution, 3D rendering).

The major objectives of the centre are:

  • Set up, maintain and develop optical technology for basic and advanced research
  • Directly provide sample acquisition and analysis in specific applications for the European Institute of Oncology and IFOM researchers
  • Provide on-site training and assistance to prepare biomedical researchers to the use of optical instrumentation and to introduce to advanced imaging applications
  • Perform independent and collaborative research for the development of advanced imaging- based technology

Activities

  • Flow Cytometry and Cell Sorting
  • Cell Microinjection
  • Wide Field Fluorescence Microscopy
  • Structured Illumination and Deconvolution
  • Confocal Microscopy
  • Live Cell Imaging (Time-Lapse Microscopy)
  • Total Internal Reflection Fluorescence (TIRF) Microscopy
  • Advanced Imaging Applications: Fluorescence Recovery After Photobleaching (FRAP), Fluorescence Resonance Energy Transfer (FRET), Photoactivation, Spectral Imaging
  • Image Cytometry and Image-based Phenotype Screening
  • Two Photon Microscopy (in collaboration with the LAMBS MicroScoBio laboratories of Prof. A Diaspro in Genoa)
  • Development of Image Analysis Software Tools
more info about this unit:
last update: February 14, 2012 . Copyright © IFOM & IEO . Campus IFOM-IEO . Via Adamello 16 . 20139 Milan Italy
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