IFOM Centre of Cell Oncology and Ultrastructure

Selection of recent publications on

Development of innovative correlative imaging approaches

• Cortese K., Diaspro A., Tacchetti C.
  Advanced Correlative Light/Electron Microscopy: Current Methods and New Developments Using Tokuyasu Cryosections.
  J. Histochem. Cytochem.; 2009 IN PRESS
• Vicidomini G, Gagliani MC, Cortese K, Krieger J, Buescher P, Bianchini P, Boccacci P, Tacchetti C, Diaspro A.
  A novel approach for correlative light electron microscopy analysis.
  Microsc Res Tech. 2009 Sep 1.
• Vicidomini G., Gagliani M.C., Canfora M., Cortese K., Frosi F., Santangelo C., Di Fiore P.P., Boccacci P., Diaspro A., and Tacchetti C.
  High data output automated 3D correlative light-electron microscopy method.
  Traffic 9:1-11; 2008

RTK internalization

• Tosoni D., Puri C., Confalonieri S., Salcini A.E., De Camilli P., Tacchetti C., and Di Fiore P.P.
  TTP specifically regulates the internalization of the transferrin receptor.
  Cell 123(5):875-88; 2005
• Fabbri M., Di Meglio S., Gagliani M. C., Consonni E., Molteni R., Bender J. R., Tacchetti C. and Pardi R.
  Dynamic partitioning into lipid rafts controls the endo-exocytic cycle of the αL/β2 integrin (LFA-1) during leukocyte chemotaxis.
  Mol Biol Cell.; 16(12):5793-803; 2005
• Puri C., Tosoni D., Comai R., Rabellino A., Segat D., Caneva F., Luzzi P., Di Fiore P.P., and Tacchetti C.
  Relationships between EGFR signaling-competent and endocytosis-competent membrane microdomains.
  Mol. Biol. Cell, 16:2704-2718; 2005 .
• Sigismund S., Woelk T., Puri C., Maspero E., Tacchetti C., Transidico P., Di Fiore P.P and Polo S.
  Clathrin-independent endocytosis of ubiquitinated cargos.
  Proc. Natl. Acad. Sci. USA, 102:2760-2765; 2005.
• Valabrega C., Montemurro F., Sarotto I., Petrelli A., Rubini P., Tacchetti C., Aglietta M., Comoglio P.M., and Giordano S.
  TGFα expression impairs Trastuzumab-induced HER2 down-regulation.
  Oncogene, 24:3002-3010; 2005.
• Nikolopoulos S. N., Blaikie P., Yoshioka T., Guo W., Puri C., Tacchetti C., and Giancotti F. G. Targeted
  Deletion of the Integrin β4 Signaling Domain Suppresses Laminin-5-Dependent Nuclear Entry of Mitogen-Activated Protein Kinases and NF-κB, Causing Defects in Epidermal Growth and Migration.
  Mol. Cell. Biol. 25: 6090-6102; 2005 .
• Croce A., Cassata G., Disanza A., Gagliani M.C., Tacchetti C., Malabarba M.G., Carlier M-F., Scita G., Baumeister R., and Di Fiore P.P.
  A novel actin barbed end-capping activity in EPS-8 regulates apical morphogenesis in intestinal cells of Caenorhabditis elegans.
  Nature Cell Biol. 6:1173-1179; 2004 .
• Orsini F, Migliaccio E, Moroni M, Contursi C, Raker VA, Piccini D, Martin-Padura I, Pelliccia G, Trinei M, Bono M, Puri C, Tacchetti C, Ferrini M, Mannucci R, Nicoletti I, Lanfrancone L, Giorgio M, Pelicci P.
  The life span determinant p66Shc localizes to mitochondria where it associates with mtHsp70 and regulates trans-membrane potential.
  J. Biol. Chem., 279:25689-25695; 2004 .
• Moro L., Dolce L., Cabodi S., Bergatto E., Boeri Erba E., Smeriglio M., Turco E., Retta F., Giuffrida G., Venturino M., Godovac-Zimmermann J., Schaefer E., Beguinot L., Tacchetti C., Gaggini P., Silengo L., Tarone G., and Defilippi P.
  Integrin-induced EGF receptor activation requires c-Src and p130Cas and leads to phosphorylation of specific EGF r eceptor tyrosines.
  J. Biol. Chem. 277:9405-9414.; 2002 .
• Salcini A.E., Hilliard M.A., Croce A., Arbucci S., Luzzi P., Tacchetti C., Daniell L., de Camilli P., Pelicci P.G., Di Fiore P.P., and Bazzicalupo P.
  EHS-1, a C. elegans homologue of Eps15, acts in the Dynamin pathway for synaptic vesicles recycling.
  Nature Cell Biol.3:755-760; 2001 .
• Mitchell K.J., Pinton P., Varadi A., Tacchetti C., Ainscow E.K., Pozzan T., Rizzuto R., and Rutter G.A.
  Dense core secretory vesicles revealed as a dynamic Ca 2+ store in neuroendocrine cells with a VAMP.aequorin chimaera.
  J. Cell Biol. 155:41-51; 2001 .
• Confalonieri S., Salcini A.E., Puri C., Tacchetti C., and Di Fiore P.P.
  Tyrosine phosphorylation of Eps15 is required for ligand-regulated bu not for constitutive endocytosis.
  J. Cell Biol.150:905-911; 2000 .
• Santolini E., Puri C., Salcini A.E., Gagliani C, Pelicci P.G., Tacchetti C. and Di Fiore P.P.
  Numb is an endocytic protein.
  J. Cell Biol. 151:1345-1351; 2000

Melanosome biogenesis

• Palmisano I., Bagnato P., Palmigiano A., Innamorati G., Rotondo G., Altimare D., Venturi C., Sviderskaya E.V., Piccirillo R., Coppola M., Marigo V., Incerti B., Ballabio A., Surace E.M., Tacchetti C., Bennett D.C., and Schiaffino M.V.
  The Ocular Albinism Type 1 (Oa1) Protein, an intracellular G Protein-Coupled receptor, regulates melanosome transport in pigment cells.
  Hum. Mol. Genet. 17:3487-501; 2008
• Cortese K., Giordano F., Surace E.M., Venturi C., Ballabio A., Tacchetti C., and Marigo V.
  The Ocular albinism type 1 (OA1) gene controls melanosome maturation and size.
  Invest. Ophthalmol. Vis. Sci. 46:4358-4364;2005
• Surace E.M., Domenici L., Cortese K., Cotugno G., Di Vicino U., Venturi C., Cellerino A., Marigo V., Tacchetti C., Ballabio A. and Auricchio A.
  Rescue of both functional and morphological abnormalities in the retina of a mouse model of ocular albinism following AAV-mediated gene transfer.
  Mol. Ther. 12:652-658; 2005
• Schiaffino M.V., and Tacchetti C.
  The Ocular Albinism Type 1 (OA1) Protein and the Evidence for an Intracellular Signal Transduction System Involved i In Melanosome Biogenesis.
  Hum. Gene Ther. 13:947-957; 2002
• Schiaffino M.V., Dellambra E., Cortese K., Baschirotto C., Bondanza S., Clementi M., Nucci P., Ballabio A., Tacchetti C., and De Luca M.
  Effective retroviral-mediated gene transfer in normal and mutant human melanocytes.
  Hum. Gene Ther. 13:947-957; 2002
• Incerti B., Cortese K., Pizzigoni A., Surace E.M., Varani S., Coppola M., Jeffery G., Seeliger M., Jaissle G., Huffstadt U., Marigo V., Schiaffino M.V., Tacchetti C., and Ballabio A.
  Oa1 knock out:new inside on the pathogenesis of Ocular Albinism type I.
  Hum. Mol. Genet. 9:2781-2788; 2000
• Schiaffino M.V., D'Addio M., Alloni A., Baschirotto C., Valetti C., Cortese K., Puri C., Bassi M.T., Colla C., Luca M., Tacchetti C. and Ballabio A.
  Ocular Albinism: evidence for a defect in an intracellular signal transduction system.
  Nature Genet. 23:108-112; 1999
• Schiaffino M.V., Baschirotto C., Pellegrini G., Montalti S., Tacchetti C., De Luca M., and Ballabio A.
  The Ocular Albinism type 1 (OA1) gene product is a membrane glycoprotein localized to melanosomes.
  Proc. Natl. Acad. Sci. USA 93:9055-9060; 1996