Membrane and actin dynamics in the control of migratory and invasive strategies

Selected Publications: Top 10 articles as Senior/Corresponding Author

• M. Hertzog, F. Milanesi, L. Hazelwood, A. Disanza, H. Liu, E. Perlade, M. Malabarba, S. Pasqualato, A. Maiolica, S. Confalonieri, C. Le Clainche, N. Offenhauser, J. Block, K. Rottner, P. P. Di Fiore, M.-F. Carlier, N. Wolkmann, D. Hanein, G. Scita, 2010.
  Molecular basis for the dual function of Eps8 on actin dynamics: bundling and capping. Plos Biology. June 8, e1000387. IF= 12.683 Citation=0
• E. Menna, A. Disanza, C. Cagnoli, U. Schenk, G. Gelsomino, E. Frittoli, M. Hertzog, N. Offenhauser, C. Sawallisch, H. J. Kreienkamp, F. B. Gertler, P. P. Di Fiore, G. Scita, M. Matteoli, 2009.
  Eps8 regulates axonal filopodia in hippocampal neurons in response to brain-derived neurotrophic factor (BDNF).
  PLoS Biol. 7, e1000138. *Corresponding author. IF=12.683 Citation=9
• Palamidessi, A. Frittoli, E. Faretta, M. Diaspro, A. Lanzetti, L. Scita, G.* and Di Fiore, P.P.
  Endocytic trafficking of Rac is required for the spatial restriction of signaling in cell migration.
  Cell (2008). Jul 11;134(1):18-20. *Corresponding author IF=31.253 Citation=112
  New & Views commentary for this article:
  -T. Zech and L. Machesky -Cell. 2008 Jul 11;134(1):18-20
  -C. Marshall-Faculty 1000 Evaluated on 23 Jul 2008
  -K. Baumann Nature Reviews Cancer 8, 662-663 (September 2008)
• Disanza A, Mantoani S, Hertzog M, Gerboth S, Frittoli E, Steffen A, Berhoerster K, Kreienkamp HJ, Milanesi F, Di Fiore PP, Ciliberto A, Stradal TE, Scita G. 2006.
  Regulation of cell shape by Cdc42 is mediated by the synergic actin-bundling activity of the Eps8-IRSp53 complex.
  Nat Cell Biol 8:1337-1347. IF=17.774 Citation=54
• Innocenti M, Gerboth S, Rottner K, Lai FP, Hertzog M, Stradal TE, Frittoli E, Didry D, Polo S, Disanza A, Benesch S, Di Fiore PP, Carlier MF, Scita G.. (2005).
  Abi1 regulates the activity of N-WASP and WAVE in distinct actin-based processes.
  Nat Cell Biol 7, 969-976 (2005). IF=17.774 Citation=84
• Andrea Disanza, Marie-France Carlier, Theresia E. B. Stradal Dominique Didry, Emanuela Frittoli, Stefano Confalonieri, Assunta Croce, Jurgen Wehland, Pier Paolo Di Fiore, and Giorgio Scita.
  Eps8 controls actin-based motility by capping the barbed ends of actin filaments.
  Nat Cell Biol 6, 1180-8 (2004). IF=17.774 Citation=67
  New & Views commentary for this article
  -H.N. Higgs Nature Cell Biology 6, 1147 - 1149 (2004)
  -Nature Cell Biology Cover (Nat Cell Biol 6, 1180-8 (2004)
• Sini, P.Cannas, A.Koleske, A. J.Di Fiore, P. P., Scita, G. Abl-dependent tyrosine phosphorylation of Sos-1 mediates growth-factor-induced Rac activation.
  Nat Cell Biol 6 (3), 268-274, (2004). IF=17.774 Citation=87
• Metello Innocenti, Adriana Zucconi, Andrea Disanza, Emanuela Frittoli, Liliana Areces, Anika Steffen, Theresia E. B. Stradal, Pier Paolo Di Fiore, Marie-France Carlier,
  and G. Scita.
  Abi1 is essential for the formation and activation of a WAVE2 signalling complex.
  Nat Cell Biol 6 (4), 319-327. (2004) IF=17.774 Citation=189
• M. Innocenti, E. Frittoli, I. Ponzanelli, J.R. Falck, S.M. Brachmann, P.P. Di Fiore, and G. Scita. 2003.
  Phosphoinositide 3-kinase activates Rac by entering in a complex with Eps8, Abi1, and Sos-1.
  J Cell Biol. 160 (1): 17-23. IF=9.52 Citation=115
• Innocenti, M., P. Tenca, E. Frittoli, M. Faretta, A. Tocchetti, P.P. Di Fiore, and G. Scita. 2002.
  Mechanisms through which Sos-1 coordinates the activation of Ras and Rac.
  J Cell Biol. 156:125-136. IF=9.52 Citation=93

5 Top "review" articles

• G. Scita and P.P. Di Fiore.
  The Endocytic Matrix.
  Nature. Jan 28, 2010. IF=31.434 Citation=0
• Scita, G., Confalonieri, S., Lappalainen, P. & Suetsugu, S.
  IRSp53: crossing the road of membrane and actin dynamics in the formation of membrane protrusions.
  Trends Cell Biol 18, 52-60 (2008). IF=13.385 Citation=29
• Stradal, T.E. & Scita, G.
  Protein complexes regulating Arp2/3-mediated actin assembly.
  Curr Opin Cell Biol (2005). Feb;18(1):4-10. 2006. IF=12.543 Citation=1
• Theresia Stradal, Klemens Rottner, Andrea Disanza, Stefano Confalonieri, Metello Innocenti and Giorgio Scita.
  Regulation of actin dynamics by WASP and WAVE family proteins. 2004.
  Trends in Cell Biology. Jun;14(6):303-11. IF=13.385 Citation=132
• Scita, G., Tenca, P., Frittoli, E., Tocchetti, A., Innocenti, M., Giardina, G., and Di Fiore, P. P. (2000).
  Signaling from Ras to Rac and beyond: not just a matter of GEFs.
  Embo J 19, 2393-8. IF=8.295 Citation=159

Publications (2004-present)

2011

• Vaggi, F., Disanza, A., Milanesi, F., Di Fiore P.P., Menna, E., Matteoli, M., Gov, N., Scita, G.*, and Ciliberto, A*. 2011.
  The Eps8/IRSp53/VASP network differentially controls actin capping and bundling in filopodia formation. PlosComBio. In press
  *Corresponding Author/Equal Contribution
• Peleg B, Disanza A, Scita G, Gov N.
  Propagating cell-membrane waves driven by curved activators of actin polymerization
  PLoS One. 2011 Apr 21;6(4):e18635.
• De Laurentiis, A., Pardo, O. E., Palamidessi, A., Jackson, S. P., Schoenwaelder, S. M., Reichmann, E., Scita, G., Arcaro, A., 2011.
  The catalytic class I(A) PI3K isoforms play divergent roles in breast cancer cell migration.
  Cell Signal. 23, 529-41.
• Manor, U., Disanza, A., Grati, M., Andrade, L., Lin, H., Di Fiore, P. P., Scita, G*., Kachar, B*., 2011.
  Regulation of stereocilia length by myosin XVa and whirlin depends on the actin-regulatory protein Eps8. Curr Biol. 21, 167-72.
  *Corresponding Author/Equal Contribution

2010

• M. Hertzog, F. Milanesi, L. Hazelwood, A. Disanza, H. Liu, E. Perlade, M. Malabarba, S. Pasqualato, A. Maiolica, S. Confalonieri, C. Le Clainche, N. Offenhauser, J. Block, K. Rottner, P. P. Di Fiore, M.-F. Carlier, N. Wolkmann, D. Hanein, G. Scita, 2010.
  Molecular basis for the dual function of Eps8 on actin dynamics: bundling and capping.
  Plos Biology. June 8, e1000387.
• A. Tocchetti, C. B. Soppo, F. Zani, F. Bianchi, M. C. Gagliani, B. Pozzi, J. Rozman, R. Elvert, N. Ehrhardt, B. Rathkolb, C. Moerth, M. Horsch, H. Fuchs, V. Gailus-Durner, J. Beckers, M. Klingenspor, E. Wolf, M. Hrabe de Angelis, E. Scanziani, C. Tacchetti, G. Scita, P. P. Di Fiore, N. Offenhauser,
  Loss of the actin remodeler Eps8 causes intestinal defects and improved metabolic status in mice.
  PLoS One. 5, e9468.
• T. Welsch, A. Younsi, A. Disanza, J. A. Rodriguez, A. M. Cuervo, G. Scita, J. Schmidt. 2010.
  Eps8 is recruited to lysosomes and subjected to chaperone-mediated autophagy in cancer cells.
  Exp Cell Res. Feb. 22, 1090-2422 (Epub).
• G. Scita, P. P. Di Fiore. 2010.
  The endocytic matrix.
  Nature. 463, 464-73 Corresponding author

2009

• B. Chauhan, A. Disanza, SY. Choi, M. Lou, H. E. Beggs, G. Scita, Y. Zheng, and RA Lang. 2009.
  Cdc42 and IRSp53-dependent contractile filipodia tether presumptive lens and retina to coordinate epithelial invagination.
  Development. In press.
• C Giuliani, F Troglio, A. Disanza, M.Soto, J Hardin, B.D. Grant and G Scita 2009.
  Requirements for F-BAR proteins TOCA-1 and TOCA-2 in actin dynamics and membrane trafficking during C. elegans oocyte growth and embryonic epidermal morphogenesis.
  Plos Genetics-In press
• A. Disanza, E. Frittoli, A. Palamidessi, G. Scita, 2009.
  Endocytosis and spatial restriction of cell signaling. Mol Oncol.
• E. Menna, A. Disanza, C. Cagnoli, U. Schenk, G. Gelsomino, E. Frittoli, M. Hertzog, N. Offenhauser, C. Sawallisch, H. J. Kreienkamp, F. B. Gertler, P. P. Di Fiore, G. Scita, M. Matteoli, 2009.
  Eps8 regulates axonal filopodia in hippocampal neurons in response to brain-derived neurotrophic factor (BDNF).
  PLoS Biol. 7, e1000138 *Corresponding author
• Yang, C., Hoelzle, M., Disanza, A., Scita, G., and Svitkina, T. (2009).
  Coordination of membrane and actin cytoskeleton dynamics during filopodia protrusion. PLoS ONE 4, e5678
• C. Ferrai, G. Naum-Ongania, E. Longobardi, M. Palazzolo, A. Disanza, V. M. Diaz, M. P. Crippa, G. Scita, F. Blasi, 2009.
  Induction of HoxB Transcription by Retinoic Acid Requires Actin Polymerization.
  Mol Biol Cell.
• A. Palamidessi, I. Testa, E. Frittoli, S. Barozzi, M. Garre, D. Mazza, P. P. Di Fiore, A. Diaspro, G. Scita, M. Faretta, 2009b.
  Understanding biological dynamics: following cells and molecules to track functions and mechanisms.
  Eur Biophys J.
• S. M. Weiss, M. Ladwein, D. Schmidt, J. Ehinger, S. Lommel, K. Stading, U. Beutling, A. Disanza, R. Frank, L. Jansch, G. Scita, F. Gunzer, K. Rottner, T. E. Stradal, 2009.
  IRSp53 links the enterohemorrhagic E. coli effectors Tir and EspFU for actin pedestal formation.
  Cell Host Microbe. 5, 244-58.
• C. Sawallisch, K. Berhorster, A. Disanza, S. Mantoani, M. Kintscher, L. Stoenica, A. Dityatev, S. Sieber, S. Kindler, F. Morellini, M. Schweizer, T. M. Boeckers, M. Korte, G. Scita, H. J. Kreienkamp, 2009.
  The insulin receptor substrate of 53 kDa (IRSp53) limits hippocampal synaptic plasticity.
  J Biol Chem. Electronic Publication: 2009/02/12
• C. Sawallisch, K. Berhorster, A. Disanza, S. Mantoani, M. Kintscher, L. Stoenica, A. Dityatev, S. Sieber, S. Kindler, F. Morellini, M. Schweizer, T. M. Boeckers, M. Korte, G. Scita, H. J. Kreienkamp, 2009.
  The insulin receptor substrate of 53 kDa (IRSp53) limits hippocampal synaptic plasticity.
  J Biol Chem. Electronic Publication: 2009/02/12

2008

• Disanza, A., and G. Scita. 2008.
  Cytoskeletal regulation: coordinating actin and microtubule dynamics in membrane trafficking.
  Curr Biol. 18:R873-5
• Palamidessi, A, Frittoli, E. Faretta, M. Diaspro , A, Letizia Lanzetti, Scita, G.*and Di Fiore, P.P. Endocytic trafficking of Rac is required for the spatial restriction of signaling in cell migration. Cell (2008). In press. *Corresponding author
• E. Frittoli, A. Palamidessi, A. Pizzigoni, L. Lanzetti, M. Garre, F. Troglio, A. Troilo, M. Fukuda, P. P. Di Fiore, G. Scita, S*. Confalonieri, 2008.
  The Primate-specific Protein TBC1D3 is Required for Optimal Macropinocytosis in a Novel ARF6 Dependent Pathway.
  Mol Biol Cell. *Corresponding author
• Scita, G., Confalonieri, S., Lappalainen, P. & Suetsugu, S.
  IRSp53: crossing the road of membrane and actin dynamics in the formation of membrane protrusions.
  Trends Cell Biol 18, 52-60 (2008). *Corresponding author

2007

• Yang C, Czech L, Gerboth S, Kojima S, Scita G, Svitkina T. (2007)
  Novel roles of formin mDia2 in lamellipodia and filopodia formation in motile cells.
  PLoS Biol 5: e317
• Bosse T, Ehinger J, Czuchra A, Benesch S, Steffen A, Wu X, Schloen K, Niemann HH, Scita G, Stradal TE, Brakebusch C, Rottner K. 2007.
  Cdc42 and PI3K drive Rac-mediated actin polymerization downstream of c-Met in distinct and common pathways.
  Mol Cell Biol 27(19):6615-28

2006

• Miyoshi T, Tsuji T, Higashida C, Hertzog M, Fujita A, Narumiya S, Scita G, Watanabe N.
  Actin turnover-dependent fast dissociation of capping protein in the dendritic nucleation actin network: evidence of frequent filament severing.
  J Cell Biol 175:947-955.
• Disanza A, Mantoani S, Hertzog M, Gerboth S, Frittoli E, Steffen A, Berhoerster K, Kreienkamp HJ, Milanesi F, Di Fiore PP, Ciliberto A, Stradal TE, Scita G. 2006.
  Regulation of cell shape by Cdc42 is mediated by the synergic actin-bundling activity of the Eps8-IRSp53 complex.
  Nat Cell Biol 8:1337-1347.
• Offenhauser, N., Offenhauser, N. Castelletti, D., Mapelli, L., Soppo, B. E., Regondi, M. C., Rossi, P., D'Angelo, E., Frassoni, C., Amadeo, A., Tocchetti, A., Pozzi, B., Disanza, A., Guarnieri, D., Betsholtz, C., Scita, G., Heberlein, U., Di Fiore, P. P..
  Increased ethanol resistance and consumption in eps8 knockoutmice correlates with altered actin dynamics.
  Cell 127, 213-226 (2006).
• Goicoechea S, Arneman D, Disanza A, Garcia-Mata R, Scita G, Otey CA
  Palladin binds to Eps8 and enhances the formation of dorsal ruffles and podosomes in vascular smooth muscle cells.
  J Cell Sci. 2006 Aug 15;119(Pt 16):3316-24. Epub 2006 Jul 25
• R. M. Katso, O. E. Pardo, A. Palamidessi, C. M. Franz, M. Marinov, A. De Laurentiis, J. Downward, G. Scita, A. J. Ridley, M. D. Waterfield, A. Arcaro, 2006b.
  Phosphoinositide 3-Kinase C2beta regulates cytoskeletal organization and cell migration via Rac-dependent mechanisms.
  Mol Biol Cell. 17, 3729-44.
• Khanday, F. A., Santhanam, L., Kasuno, K., Yamamori, T., Naqvi, A., Dericco, J., Bugayenko, A., Mattagajasingh, I., Disanza, A., Scita, G.,
  Irani, K.. Sos-mediated activation of rac1 by p66shc.
  J Cell Biol. Mar 6 2006.

2005

• Stradal, T.E. & Scita, G.
  Protein complexes regulating Arp2/3-mediated actin assembly.
  Curr Opin Cell Biol (2005). Feb;18(1):4-10. 2006.
• Innocenti M, Gerboth S, Rottner K, Lai FP, Hertzog M, Stradal TE, Frittoli E, Didry D, Polo S, Disanza A, Benesch S, Di Fiore PP, Carlier MF, Scita G. (2005).
  Abi1 regulates the activity of N-WASP and WAVE in distinct actin-based processes.
  Nat Cell Biol 7, 969-976 (2005).
• H. Bierne, H. Miki, M. Innocenti, G. Scita, F. B. Gertler, T. Takenawa, P. Cossart, 2005.
  WASP-related proteins, Abi1 and Ena/VASP are required for Listeria invasion induced by the Met receptor.
  J Cell Sci. 118, 1537-47.
• Shi, J., Scita, G., and Casanova, J. E. (2005).
  WAVE2 Signaling Mediates Invasion of Polarized Epithelial Cells by Salmonella typhimurium.
  J Biol Chem 280, 29849-29855

2004

• Andrea Disanza, Marie-France Carlier, Theresia E. B. Stradal Dominique Didry, Emanuela Frittoli,, Stefano Confalonieri, Assunta Croce, Jurgen Wehland, Pier Paolo Di Fiore, and Giorgio Scita.
  Eps8 controls actin-based motility by capping the barbed ends of actin filaments.
  Nat Cell Biol 6, 1180-8 (2004).
• Assunta Croce, Giuseppe Cassata Andrea Disanza, Maria Cristina Gagliani, Carlo Tacchetti, Maria Grazia Malabarba, Marie-France Carlier, Giorgio Scita1, Ralf Baumeister3 and Pier Paolo Di Fiore.
  A novel actin barbed end-capping activity in EPS-8 regulates apical morphogenesis in intestinal cells of Caenorhabditis elegans.
  Nat Cell Biol 6, 1173-9 (2004).
• Letizia Lanzetti, Andrea Palamidessi, Liliana Areces, Giorgio Scita and Pier Paolo Di Fiore. Rab5 is a signalling GTPase involved in actin remodelling by receptor tyrosine kinases. 2004. Nature.429 : 309-314.
• Sini, P.Cannas, A.Koleske, A. J.Di Fiore, P. P., Scita, G.
  Abl-dependent tyrosine phosphorylation of Sos-1 mediates growth-factor-induced Rac activation.
  Nat Cell Biol 6 (3), 268-274.
• Metello Innocenti, Adriana Zucconi, Andrea Disanza, Emanuela Frittoli, Liliana Areces, Anika Steffen, Theresia E. B. Stradal, Pier Paolo Di Fiore, Marie-France Carlier, and G. Scita.
  Abi1 is essential for the formation and activation of a WAVE2 signalling complex. 2004.
  Nat Cell Biol 6 (4), 319-327.