Epigenetic mechanisms in stem cell differentiation and oncogenesis

Selected publications

• Vella, P., Barozzi, I., Cuomo, A., Bonaldi, T. & Pasini, D. (2011)
  Yin Yang 1 extends the Myc-related transcription factors network in embryonic stem cells.
  Nucleic Acids Res, doi:10.1093/nar/gkr1290
• Stojic, L., Jasencakova, Z., Prezioso, C., Stutzer, A., Bodega, B., Pasini, D., Klingberg, R., Mozzetta, C., Margueron, R., Puri, P.L., Schwarzer, D., Helin, K., Fischle, W. and Orlando, V. (2011)
  Chromatin regulated interchange between polycomb repressive complex 2 (PRC2)-Ezh2 and PRC2-Ezh1 complexes controls myogenin activation in skeletal muscle cells.
  Epigenetics Chromatin, 4, 16.
• Piunti, A. and Pasini, D. (2011)
  Epigenetic factors in cancer development: polycomb group proteins.
  Future Oncol, 7, 57-75.
• Pasini, D., Malatesta, M., Jung, H.R., Walfridsson, J., Willer, A., Olsson, L., Skotte, J., Wutz, A., Porse, B., Jensen, O.N. and Helin, K. (2010)
  Characterization of an antagonistic switch between histone H3 lysine 27 methylation and acetylation in the transcriptional regulation of Polycomb group target genes.
  Nucleic Acids Res, 38, 4958-4969.
• Pasini, D., Cloos, P.A., Walfridsson, J., Olsson, L., Bukowski, J.P., Johansen, J.V., Bak, M., Tommerup, N., Rappsilber, J. and Helin, K. (2010)
  JARID2 regulates binding of the Polycomb repressive complex 2 to target genes in ES cells.
  Nature, 464, 306-310.
• Leeb, M., Pasini, D., Novatchkova, M., Jaritz, M., Helin, K. and Wutz, A. (2010)
  Polycomb complexes act redundantly to repress genomic repeats and genes.
  Genes Dev, 24, 265-276.
• Jung, H.R., Pasini, D., Helin, K. and Jensen, O.N. (2010)
  Quantitative mass spectrometry of histones H3.2 and H3.3 in Suz12-deficient mouse embryonic stem cells reveals distinct, dynamic post-translational modifications at Lys-27 and Lys-36.
  Mol Cell Proteomics, 9, 838-850.
• Riising, E.M., Boggio, R., Chiocca, S., Helin, K. and Pasini, D. (2008)
  The polycomb repressive complex 2 is a potential target of SUMO modifications.
  PLoS One, 3, e2704.
• Pasini, D., Hansen, K.H., Christensen, J., Agger, K., Cloos, P.A. and Helin, K. (2008)
  Coordinated regulation of transcriptional repression by the RBP2 H3K4 demethylase and Polycomb-Repressive Complex 2.
  Genes Dev, 22, 1345-1355.
• Pasini, D., Bracken, A.P., Agger, K., Christensen, J., Hansen, K., Cloos, P.A. and Helin, K. (2008)
  Regulation of stem cell differentiation by histone methyltransferases and demethylases.
  old Spring Harb Symp Quant Biol, 73, 253-263.
• Lindroth, A.M., Park, Y.J., McLean, C.M., Dokshin, G.A., Persson, J.M., Herman, H., Pasini, D., Miro, X., Donohoe, M.E., Lee, J.T., Helin, K. and Soloway, P.D. (2008)
  Antagonism between DNA and H3K27 methylation at the imprinted Rasgrf1 locus.
  PLoS Genet, 4, e1000145.
• Herranz, N., Pasini, D., Diaz, V.M., Franci, C., Gutierrez, A., Dave, N., Escriva, M., Hernandez-Munoz, I., Di Croce, L., Helin, K., Garcia de Herreros, A. and Peiro, S. (2008)
  Polycomb complex 2 is required for E-cadherin repression by the Snail1 transcription factor.
  Mol Cell Biol, 28, 4772-4781.
• Hansen, K.H., Bracken, A.P., Pasini, D., Dietrich, N., Gehani, S.S., Monrad, A., Rappsilber, J., Lerdrup, M. and Helin, K. (2008)
  A model for transmission of the H3K27me3 epigenetic mark.
  Nat Cell Biol, 10, 1291-1300.
• Villa, R., Pasini, D., Gutierrez, A., Morey, L., Occhionorelli, M., Vire, E., Nomdedeu, J.F., Jenuwein, T., Pelicci, P.G., Minucci, S., Fuks, F., Helin, K. and Di Croce, L. (2007)
  Role of the polycomb repressive complex 2 in acute promyelocytic leukemia.
  Cancer Cell, 11, 513-525.
• Pasini, D., Bracken, A.P., Hansen, J.B., Capillo, M. and Helin, K. (2007)
  The polycomb group protein Suz12 is required for embryonic stem cell differentiation.
  Mol Cell Biol, 27, 3769-3779.
• Christensen, J., Agger, K., Cloos, P.A., Pasini, D., Rose, S., Sennels, L., Rappsilber, J., Hansen, K.H., Salcini, A.E. and Helin, K. (2007)
  RBP2 belongs to a family of demethylases, specific for tri-and dimethylated lysine 4 on histone 3.
  Cell, 128, 1063-1076.
• Bracken, A.P., Kleine-Kohlbrecher, D., Dietrich, N., Pasini, D., Gargiulo, G., Beekman, C., Theilgaard-Monch, K., Minucci, S., Porse, B.T., Marine, J.C., Hansen, K.H. and Helin, K. (2007)
  The Polycomb group proteins bind throughout the INK4A-ARF locus and are disassociated in senescent cells.
  Genes Dev, 21, 525-530.
• Agger, K., Cloos, P.A., Christensen, J., Pasini, D., Rose, S., Rappsilber, J., Issaeva, I., Canaani, E., Salcini, A.E. and Helin, K. (2007)
  UTX and JMJD3 are histone H3K27 demethylases involved in HOX gene regulation and development.
  Nature, 449, 731-734.
• Bracken, A.P., Dietrich, N., Pasini, D., Hansen, K.H. and Helin, K. (2006)
  Genome-wide mapping of Polycomb target genes unravels their roles in cell fate transitions.
  Genes Dev, 20, 1123-1136.
• Lazzerini Denchi, E., Attwooll, C., Pasini, D. and Helin, K. (2005)
  Deregulated E2F activity induces hyperplasia and senescence-like features in the mouse pituitary gland.
  Mol Cell Biol, 25, 2660-2672.
• Pasini, D., Bracken, A.P., Jensen, M.R., Lazzerini Denchi, E. and Helin, K. (2004)
  Suz12 is essential for mouse development and for EZH2 histone methyltransferase activity.
  EMBO J, 23, 4061-4071.
• Pasini, D., Bracken, A.P. and Helin, K. (2004)
  Polycomb group proteins in cell cycle progression and cancer.
  Cell Cycle, 3, 396-400.
• Danovi, D., Meulmeester, E., Pasini, D., Migliorini, D., Capra, M., Frenk, R., de Graaf, P., Francoz, S., Gasparini, P., Gobbi, A., Helin, K., Pelicci, P.G., Jochemsen, A.G. and Marine, J.C. (2004)
  Amplification of Mdmx (or Mdm4) directly contributes to tumor formation by inhibiting p53 tumor suppressor activity.
  Mol Cell Biol, 24, 5835-5843.
• Gao, G., Bracken, A.P., Burkard, K., Pasini, D., Classon, M., Attwooll, C., Sagara, M., Imai, T., Helin, K. and Zhao, J. (2003)
  NPAT expression is regulated by E2F and is essential for cell cycle progression.
  Mol Cell Biol, 23, 2821-2833.
• Bracken, A.P.*, Pasini, D.*, Capra, M., Prosperini, E., Colli, E. and Helin, K. (2003)
  EZH2 is downstream of the pRB-E2F pathway, essential for proliferation and amplified in cancer. EMBO J, 22, 5323-5335.
  *authors equal contribution