Microscopy and Nanoscopy for 3D tracking and visualization of biological molecules in cellular systems by means of linear and non-linear optical interactions

Selected publications

A) Towards Single Molecule 4D Tracking and Nanoscopy.

• CAORSI V, RONZITTI E, VICIDOMINI G, KROL S, MCCONNELL G, DIASPRO A. (2007).
  FRET measurements on fuzzy fluorescent nano-structures.
  MICROSC. RES. TECH. vol. 70, in press.
• VICIDOMINI G, SCHNEIDER M, BIANCHINI P, KROL S, SZELLAS T, DIASPRO A. (2007).
  Characterization of uniform ultrathin layer for z-response measurements in three-dimensional section fluorescence microscopy.
  J. MICROSC. vol. 225, pp. 88-95
• DIASPRO A., KROL S, CAMPANINI B, CANNONE F, CHIRICO G. (2006).
  Enhanced Green Fluorescent Protein (GFP) fluorescence after polyelectrolyte caging.
  OPTICS EXPRESS. vol. 14, pp. 9815-9824.
• CHIRICO G, CANNONE F, DIASPRO A. (2006).
  Unfolding time distribution of GFP by single molecule fluorescence spectroscopy.
  EUR. BIOPHYS. J. vol. 35(8), pp. 663-674.
• SCHNEIDER M, BAROZZI S, TESTA I, FARETTA M, DIASPRO A. (2005).
  Two-photon activation and excitation properties of PA-GFP in the 720-920-nm region.
  BIOPHYS. J. vol. 89(2), pp. 1346-1352.
• CHIRICO G., DIASPRO A., CANNONE F., COLLINI M., BOLOGNA S., PELLEGRINI V., BELTRAM F. (2005).
  Selective fluorescence recovery after bleaching of single E2GFP proteins induced by two-photons excitation.
  CHEMPHYSCHEM. vol. 6, pp. 328-335.
• DIASPRO A., FEDERICI F, VIAPPIANI C, KROL S, PISCIOTTA M, CHIRICO G, CANNONE F, GLIOZZI A. (2003).
  Two-photon photolysis of 2-nitrobenzaldehyde monitored by fluorescent-labeled nanocapsules.
  J. PHYS. CHEM. B. vol. 107, pp. 11008-11012
• CHIRICO G, CANNONE F, DIASPRO A. (2003).
  Single molecule photodynamics by means of one- and two-photon approach.
  J. PHYSICS D. vol. 36, pp. 1682-1688
• CHIRICO G, CANNONE F, BERETTA S, BALDINI G, DIASPRO A. (2001).
  Single molecule studies by means of the two-photon fluorescence distribution.
  MICROSC. RES. TECH. vol. 55(5), pp. 359-364.
• DIASPRO A., AGUILAR M., BIANCO B. (1993).
  Detection of displacements in the nanometer scale by optical tunneling.
  SENSORS AND ACTUATORS. A. vol. 37/38, pp. 557-581

B) Selection of Biological papers

• RAMOINO P, GALLUS L, BELTRAME F, DIASPRO A., FATO M, RUBINI P, STIGLIANI S, BONANNO G, USAI C. (2006).
  Endocytosis of GABAB receptors modulates membrane excitability in the single-celled organism Paramecium.
  JOURNAL OF CELL SCIENCE. vol. 119(10), pp. 2056-2064.
• NIZZARI M, VENEZIA V, BIANCHINI P, CAORSI V, DIASPRO A., REPETTO E, THELLUNG S, CORSARO A, SCHETTINI G, CARLO P, FLORIO T, RUSSO C. (2006).
  Amyloid precursor protein and presenilin 1 interaction studied by fret in human H4 cells.
  In: ANNALS NEW YORK ACADEMY of SCIENCES. In press.
• GALLUS L, FERRANDO S, BOTTARO M, GIROSI L, RAMOINO P, DIASPRO A., ALUIGI M.G, TAGLIAFIERRO G. (2006).
  Distribution of choline acetyltransferase immunoreactivity in the alimentary tract of the barnacle Balanus amphitrite (Cirripedia, Crustacea).
  NEUROSCIENCE LETTERS. vol. 409(3), pp. 230-233.
• STIGLIANI S, ZAPPETTINI S, RAITERI L, PASSALACQUA M, MELLONI E, VENTURI C, TACCHETTI C, DIASPRO A., USAI C, BONANNO G. (2006).
  Glia re-sealed particles freshly prepared from adult rat brain are competent for exocytotic release of glutamate.
  J. NEUROCHEM. vol. 96, pp. 656-668.
• RAMOINO P, USAI C, BELTRAME F, FATO M, GALLUS L, TAGLIAFIERRO G, MAGRASSI R, DIASPRO A. (2005).
  GABA(B) receptor intracellular trafficking after internalization in Paramecium.
  MICROSC. RES. TECH. vol. 68(5), pp. 290-295.
• FABRIZIO P., BATTISTELLA L., VARDAVAS R., GATTAZZO C., LIOU L.L., DIASPRO A., DOSSEN J.W., BUTLER GRALLA E., LONGO V.D. (2004).
  Superoxide is a mediator of an altruistic aging program in Saccharomyces cerevisiae.
  THE JOURNAL OF CELL BIOLOGY. vol. 166 (7), pp. 1055-1067.
• PECERE T, SARINELLA F, SALATA C, GATTO B, BET A, DALLA VECCHIA F, DIASPRO A., CARLI M, PALUMBO M, PALU G. (2003).
  Involvement of p53 in specific anti-neuroectodermal tumor activity of aloe-emodin.
  INT. J. CANCER. vol. 106, pp. 836-847.
• FABRIZIO P., LIQU L.L., MOY V.N., DIASPRO A., SELVERSTONE VALENTINE J., GRALLA E.B., LONGO V.D. (2003).
  SOD2 functions downstream of SCH9 to extend longevity in yeast.
  GENETICS. vol. 163, pp. 35-46.
• BARBORO P., D'ARRIGO C., DIASPRO A., MORMINO M., ALBERTI I., PARODI S., PATRONE E., BALBI C. (2002).
  Unravealing the organization of the internal nuclear matrix: rna-dependent anchoring of numa to a lamin scaffold.
  EXPER. CELL RES. vol. 279, pp. 202-218.
• BORGHI R., PELLEGRINI L., LACANA E., DIASPRO A., PRONZATO M.A., VITALI A., RONCARATI R., STROCCHI P., ZACCHEO D., D'ADAMIO L., TABATON M. (2002).
  Neuronal apoptosis is accompanied by amyloid beta-protein accumulation in the endoplasmic reticulum.
  J. ALZHEIMER'S DIS. vol. 4, pp. 31-37.
• BALDUZZI R, CUPELLO A, DIASPRO A., RAMOINO P. (2001).
  Confocal microscopic study of GABA(A) receptors in Xenopus oocytes after rat brain mRNA injection: modulation by tyrosine kinase activity.
  BBA. vol. 1539, pp. 93-100.
• RAMOINO P., FRONTE P., FATO M., BELTRAME F., DIASPRO A. (2001).
  Fluid phase and receptor-mediated endocytosis in paramecium primaurelia by fluorescence confocal laser scanning microscopy.
  EUR. BIOPHYS. J. vol. 30, pp. 305-312 I
• PECERE T., GAZZOLA M.V., MUCIGNAT C., PAROLIN C., DALLA VECCHIA F., CAVAGGIONI A., BASSO G., DIASPRO A., SALVATO B., CARLI M., PALU' G. (2000).
  Aloe-emodin is a new type of anticancer agent with selective activity against neuroectodermal tumors.
  CANCER RESEARCH. vol. 60, pp. 2800-2804.
• RAMOINO P, DIASPRO A., FATO M, BELTRAME F. (1999).
  Cytofluorometry and fluorescence confocal laser scanning microscopy (CLSM) in the study of neutral lipid dynamics in Paramecium primaurelia mating types during cell line development.
  CYTOMETRY. vol. 35, pp. 346-352
• DIASPRO A., BERTOLOTTO M., VERGANI L., NICOLINI C. (1991).
  Polarized light scattering of nucleosomes and polynucleosomes-in situ and in vitro studies.
  IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING. vol. 38(7), pp. 670-678
• DIASPRO A., NICOLINI CA. (1987).
  Circular intensity differential scattering and chromatin-DNA structure. A combined theoretical approach.
  CELL BIOPHYSICS. vol. 10, pp. 45-60.

C) Selection on Multiphoton and Confocal Microscopy (reviews, book chapters and technical papers).

• DIASPRO A., ed. (2001).
  CONFOCAL AND TWO-PHOTON MICROSCOPY: FOUNDATIONS, APPLICATIONS AND ADVANCES.
  pp. 1-567. WILEY-LISS (USA).
• DIASPRO A., SCHNEIDER M, BIANCHINI P, CAORSI V, MAZZA D, PESCE M, TESTA I, VICIDOMINI G, USAI C. (2007).
  Two-photon excitation and confocal fluorescence microscopy.
  In: HAWKES P.W., SPENCE J.C.H. Science of Microscopy. vol. 2(11), pp. 751-789) Springer (USA).
• DIASPRO A., BIANCHINI P, VICIDOMINI G, FARETTA M, RAMOINO P, USAI C. (2006).
  Multi-photon excitation microscopy.
  BIOMED. ENG. ONLINE. vol. 5, pp. 36-50.
• DIASPRO A., USAI C, CHIRICO G, RAMOINO P, DOBRUCKI J. (2006).
  Photobleaching.
  In: J. B. PAWLEY ED. Handbook of Biological Applications in Confocal Microscopy. pp. 690-702. Springer (USA).
• DIASPRO A., CHIRICO G. COLLINI M. (2005).
  Two-photon fluorescence excitation and related techniques in biological microscopy.
  QUART. REV. BIOPHYS. vol. 38(2), pp. 1-72.
• DIASPRO A. (2004).
  Confocal and Multiphoton Microscopy.
  In: PALUMBO G., PRATESI R. Lasers and Current Optical Techniques in Biology. pp. 429-480 Springer-RSC (UK).
• DIASPRO A., FEDERICI F. (2002).
  Influence of refractive-index mismatch in high-resolution three-dimensional confocal microscopy.
  APPLIED OPTICS. vol. 41, pp. 685-690.
• DIASPRO A., CHIRICO G, FEDERICI F, CANNONE F, BERETTA S. (2001).
  Two-photon microscopy and spectroscopy based on a compact confocal scanning head.
  J. BIOMED. OPT. vol. 6(3), pp. 280-290.
• DIASPRO A. (2000).
  Two-photon excitation of fluorescence for three-dimensional optical imaging of biological structures.
  J. PHOTOCHEM. PHOTOBIOL. B. vol. 55, pp. 1-8.
• DIASPRO A., COROSU M., RAMOINO P. (1999)
  Adapting a compact confocal microscope system to a two-photon excitation fluorescence imaging architecture.
  MICROSC. RES. TECH. vol. 47, pp. 196-205

D) Selection on Nanobiorobots papers

• DIASPRO A., SILVANO D., KROL S., CAVALLERI O., GLIOZZI A. (2002).
  Single living cell encapsulation in nano-organized polyelectrolyte shells.
  LANGMUIR. vol. 18, pp. 5047-5050.
• DIASPRO A. (2004).
  Nanocapsules: A European Community Interdisciplinary Network in the Nanobiosciences.
  IEEE TRANSACTIONS ON NANOBIOSCIENCE. vol. 3(1), pp. 1-2.
• KROL S, DEL GUERRA S, GRUPILLO M, DIASPRO A., GLIOZZI A, MARCHETTI P. (2006).
  Multilayer nanoencapsulation. New approach for immune protection of human pancreatic islets.
  NANO LETTERS. vol. 6(9), pp. 1933-1939.
• CHANANA M, GLIOZZI A, DIASPRO A., CHODNEVSKAJA I, HUEWEL S, MOSKALENKO V, ULRICHS K, GALLA HJ, KROL S. (2005).
  Interaction of Polyelectrolytes and Their Composites with Living Cells.
  NANO LETTERS. vol. 5(12), pp. 2605-2612.
• KROL S., NOLTE M., DIASPRO A., MAZZA D., MAGRASSI R., GLIOZZI A., FERY A. (2005).
  Encapsulated living cells on microstructured surfaces.
  LANGMUIR. vol. 21, pp. 705-709.
• KROL S., DIASPRO A., MAGRASSI R., BALLARIO P., GRIMALDI B., FILETICI P., ORNAGHI P., RAMOINO P., GLIOZZI A. (2004).
  Nanocapsules: coating for living cells.
  IEEE TRANSACTIONS ON NANOBIOSCIENCE. vol. 3(1), pp. 32-38.
• KROL S, CAVALLERI O, RAMOINO P, GLIOZZI A, DIASPRO A. (2003).
  Encapsulated yeast cells inside Paramecium primaurelia: a model system for protection capability of polyelectrolyte shells.
  JOURNAL OF MICROSCOPY. vol. 212, pp. 239-243.
• DIASPRO A., KROL S, CAVALLERI O, SILVANO D, GLIOZZI A. (2002).
  Microscopical characterization of nanocapsules templated on ionic crystals and biological cells toward biomedical applications.
  IEEE TRANSACTIONS ON NANOBIOSCIENCE. vol. 1(3), pp. 110-115.
• KROL S., NOLTE M., DIASPRO A., MAZZA D., MAGRASSI R., GLIOZZI A., FERY A. (2005).
  Encapsulated living cells on microstructured surfaces.
  LANGMUIR. vol. 21, pp. 705-709.

E) Selection on Advanced Image Restoration.

• VICIDOMINI G, MONDAL P.P, DIASPRO A. (2006).
  Fuzzy logic and maximum a posteriori-based image restoration for confocal microscopy.
  OPT. LETT. vol. 31, pp. 3582-3584
• BONETTO P., BOCCACCI P., SCARITO M., DAVOLIO M., EPIFANI M., VICIDOMINI G., TACCHETTI C., RAMOINO P., USAI C., DIASPRO A. (2004).
  Three-dimensional microscopy migrates to the web with "PowerUp Your Microscope".
  MICROSC. RES. TECH. vol. 64, pp. 196-203.
• DIFATO F., MAZZONE F., SCAGLIONE S., FATO M., BELTRAME F., KUBINOVA L., JANACECK J., RAMOINO P., DIASPRO A. (2004).
  Improvement in volume estimation from confocal sections after image deconvolution.
  MICROSC. RES. TECH. vol. 64, pp. 151-155
• DIASPRO A., FEDERICI F. (2002).
  Influence of refractive-index mismatch in high-resolution three-dimensional confocal microscopy.
  APPL. OPT. vol. 41, pp. 685-690
• DIASPRO A., ANNUNZIATA S., ROBELLO M. (2000).
  Single-pinhole confocal imaging of sub-resolution sparse objects using experimental point spread function and image restoration.
  MICROSC. RES. TECH. vol. 51, pp. 464-468.
• DIASPRO A., BELTRAME F, FATO M, PALMERI A, RAMOINO P. (1997).
  Studies on the structure of sperm heads of Eledone cirrhosa by means of CLSM linked to bioimage-oriented devices.
  MICROSC. RES. TECH. vol. 36(3), pp. 159-164.
• TOMMASI T, DIASPRO A., BIANCO B. (1993).
  3-D reconstruction in optical microscopy by a frequency-domain approach.
  SIGNAL PROCESSING. vol. 32(3), pp. 357-366
• DIASPRO A., SARTORE M, NICOLINI C. (1990).
  3D representation of biostructures imaged with an optical microscope .A: Digital optical sectioning .B: 3D graphic representation.
  IMAGE VIS. COMPUT. vol. 8(2), pp. 130-141
• DIASPRO A., ADAMI M, SARTORE M, NICOLINI C. (1990).
  IMAGO: a complete system for acquisition, processing, two/three-dimensional and temporal display of microscopic bio-images.
  COMPUTER METHODS AND PROGRAMS IN BIOMEDICINE. vol. 31, pp. 225-236.
• BIANCO B, DIASPRO A. (1989).
  Analysis of three-dimensional cell imaging obtained with optical microscopy techniques based on defocusing.
  CELL BIOPHYS. vol. 15, pp. 189-199.