c/o IFOM-IEO Campus
Via Adamello, 16 - 20139 Milan, Italy
Tel. +39 02 574303.226 - Fax +39 02 574303.231
High-throughput technologies are the most powerful tools for attempting holistic approaches in the post-genome era, and are rapidly becoming essential for the discovery and analysis of genetic networks underlying cancer. The use of microarrays and to study specific gene expression patterns in cancer model systems is a well established procedure. More recent technological advancements, including next-generation sequencing, have allowed for a more comprehensive analysis of complex molecular interactions that accompany transformation and tumor progression. The concerted use of these approaches to discover and characterize genetic and epigenetic events that are relevant to oncogenesis is one of the current challenges in the field of oncogenomics.
We are using an integrated genomic approach to study the molecular basis of acute leukemias. In particular, we are performing detailed analyses of transcriptional networks underlying normal hematopoietic differentiation and their subversion in the pathogenesis of leukemogenesis. Our approach includes the integration of data deriving from gene expression profiling, analysis of transcription factor binding sites through chromatin immunoprecipitation coupled to deep sequencing (ChIP-seq), identification of histone modifications and proteomic analysis of transcriptional complexes containing leukemia-associated oncoproteins. Furthermore, we are specifically analysing the oncogene-induced activation of stem-cell signalling pathways in leukemia.